Breeding for disease resistance is an important objective in
Brassica
crop improvement. In this study, we evaluated the applicability of previously published molecular markers associated with downy mildew resistance in
Brassica oleracea
genebank material. Phenotypic resistance data were obtained from previous inoculation experiments with
Hyaloperonospora parasitica
isolates. A subset of 313 individual plants from 115
Brassica
accessions, representing different resistance phenotypes at the cotyledon stage, was selected for molecular analysis. Five SSR markers associated with the resistance loci
Pp
523
,
Ppa
207
, and
Ppa3
were tested: CB10028, CB10139, BoGMS0900, BoGMS0486, and BoGMS0624. The SSR markers amplified 29 alleles across the analysed material. However, marker–phenotype associations were not fully consistent across crop types and accessions. The highest informativeness was observed for CB10028 and BoGMS0900, whereas other markers showed stronger dependence on genetic background. Our results indicate that published resistance-linked markers can provide useful information for
Brassica
germplasm characterization, but they cannot be used as universal predictors of downy mildew resistance without further validation in defined genetic backgrounds and against individual pathogen isolates. The present marker evaluation is limited to cotyledon-stage resistance phenotypes, and further phenotyping would be required to assess marker applicability to true-leaf or adult-plant resistance.
Michaela Jungová, Pavel Svoboda, I. Petrželová et al.· European journal of plant pa...· 0 citations
Abstract The evolution of plant genomics has been shaped by several pioneering milestones, beginning with the introduction of restriction fragment length polymorphism–based genetic linkage maps in the mid‐1980s. Among the global contributors, Prof. Chittaranjan Kole stands as a distinguished figure whose work fundamentally shifted the trajectory of plant genomics and molecular breeding. This tribute highlights his scientific journey and groundbreaking contributions, from being the first Indian scientist to physically map and sequence a plant gene in barley to establishing the foundations of molecular cytogenetics, comparative genomics, and molecular evolution and phylogenetic relationships in plants. His landmark research on Brassica genomics, including high‐resolution mapping, Mendelization of quantitative trait loci (QTLs), and innovative use of recombinant inbred lines, enabled unprecedented insights into trait evolution, stress biology, and genome homology between Brassica species and Arabidopsis. Prof. Kole's work on mapping genes and QTLs associated with flowering time, biotic stress resistance, abiotic stress tolerance, and genome evolution has provided a framework now integral to marker‐assisted selection, genomic breeding, and climate‐resilient crop development. This article offers a scholarly reflection on his pioneering contributions, establishing Prof. Kole as a founding architect of plant genomics research in India and one of its most influential contributors globally.
M. Nageswara-Rao, Sarita Pandey, Poulami Bhattacharjee et al.· The Plant Genome· 0 citations
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