#software testing
Sep 2026
Comparison of manual counting with two rule-based image analysis programs, ImageJ and CellProfiler, for quantitative analysis of c-Fos immunohistochemical positivity in rat brainstem sections.
Under the predefined user-guided threshold ranges used, both software platforms closely matched manual counting; however, image-dependent threshold adjustment remains a potential source of observer variability.
Manal Udwan, Ayman M. Al-Qaaneh, Mimas Al-Helalat et al.
· Biotechnic & Histochemistry · 0 citations