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Eugenia S. Bardossy

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Molecular Cloning and Reverse Genetics.

The protocol covers all major steps, from the initial PCRs to create the mutated insert to its digestion and ligation into the vector, and includes procedures for bacterial transformation, colony screening via PCR and Sanger sequencing, and plasmid purification via miniprep.

Eugenia S. Bardossy · Methods in molecular biology · 0 citations