Elaine M. L. Ho

Adaptive Milling: imaging feedback-driven automated fabrication of consistently thin cryo-lamellae

Cryo-electron tomography of 100-200 nm lamellae prepared by focused ion beam milling enables structural analysis of macromolecules within cells, tissues, and small organisms, but lamella preparation remains time-consuming and limits throughput. Although automated workflows enable unsupervised fabrication, they commonly...