Innovative Method for Evaluating the Optical Properties of Liquid-Liquid Phase Separations Using Laser Scanning Microscopic Spectroscopy.
Liquid-liquid phase separation (LLPS) plays a central role in intracellular compartmentalization and gene regulation. Although optogenetic optoDroplet systems are widely used to study LLPS in living cells, the mechanisms underlying the transition from dynamic liquid-like condensates to operationally defined gel-like as...