Studying Mucin-Derived O‑Glycopeptides with Gas-Phase FRET
At least 50% of human proteins are glycosylated, however, it is not fully understood how glycosylation affects glycopeptide structures. For the highly O-glycosylated tandem repeats of the gel-forming mucin MUC5AC, it has been shown that glycoclustering leads to significant stiffening of the peptide backbone in solution...