Fusion of a non-specific DNA-binding domain enhances Cas12a trans-cleavage for robust nucleic-acid diagnostics
CRISPR–Cas12a underlies powerful genome-editing and nucleic-acid detection technologies, yet its performance is limited by inefficient target engagement and low catalytic turnover, particularly at low target abundance and elevated temperatures. Here, we report a modular protein-engineering strategy to enhance Cas12a tr...
Diogo Figueiredo, Mitsuru Hattori, Tetsuichi Wazawa et al.
· Scientific Reports · 0 citations