De Yang

A Second-Shell Mutation Preorganizes HpnG for Broad-Scope Nucleoside Transglycosylation

Nucleoside analogues are important therapeutic scaffolds, but their synthesis is often protecting-group-intensive. Purine nucleoside phosphorylases offer a mild enzymatic alternative, yet broad substrate accommodation does not necessarily lead to productive glycosyl transfer. Here, we repurposed HpnG, a hopanoid-asso...