Stable transformation in rose is slow and genotype-dependent, motivating rapid cellular assays for candidate-gene testing. We systematically evaluated mesophyll protoplast isolation and PEG-mediated transient expression in Rosa chinensis ‘Old Blush’. Sequential experiments compared enzyme preparations, digestion time,...
Gene duplication with sub- and/or neo-functionalization is a key pathway in plant adaptive evolution. The duplication-degeneration-complementation (DDC) model explains sub-functionalization of paralogs, yet the relative contributions of coding sequence versus cis-regulatory changes remain contested. In this study, we s...
Haofei Wang, Ji-Fan Zhang, Ruoting Wang et al.· Plant, Cell and Environment· 0 citations
Plants balance vegetative growth and stress responses through antagonistic phytohormone crosstalk, notably between abscisic acid (ABA) and cytokinin. However, the genetic architecture and molecular mechanisms driving this adaptive trade-off, particularly in perennial trees, remain elusive.
Integrating ge...
PtomiR393a, a drought‐responsive microRNA in Populus tomentosa that mediates crosstalk between ABA and auxin signaling pathways under drought stress is identified, offering new insights into drought tolerance mechanisms in trees and suggest potential strategies to enhance forest tree resilience to water‐deficit conditi...
Yong-Ming Chen, Ming-Yang Quan, Dan Wang et al.· New Phytologist· 0 citations
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