Open access
Jul 2026
AI-redesigned starting points and outcomes enhance protein evolution
Engineered or laboratory-evolved proteins often have suboptimal stability, activity, or specificity. We applied AI-based protein sequence design to address challenges in experimental enzyme evolution. Using the model ProteinMPNN, we redesigned three distinct botulinum neurotoxin (BoNT) proteases, generating variants wi...
Nicholas A. Krasnow, Joy A Xu, E. Zhang et al.
· Nature · 2 citations