Engineering editopes through programmable RNA editing toward tumor neoantigen generation.
Short Precise-Encodable ADAR Recruiting (SPEAR) gRNAs are developed that harness endogenous ADAR1 to direct precise adenosine-to-inosine (A-to-I) editing at selected transcript sites and demonstrate the generation of immunogenic neoepitopes through RNA editing at the transcript level, termed editopes.