MAD7-mediated genome editing via Agrobacterium enables recovery of hexa-allelic edited lines carrying distinct mutations in all six gene copies in wheat (Triticum aestivum L.) T0 plants and stable inheritance of edits into the following generation
The expansion of genome-editing tools for bread wheat ( Triticum aestivum L.) is essential to accelerate trait improvement while addressing intellectual property constraints associated with widely used CRISPR systems. MAD7 (ErCas12a), a royalty-accessible CRISPR nuclease, represents a potential alternative to CRI...