A comprehensive transcriptomic and chromatin accessibility atlas of 8 brain regions of 23 female cynomolgus macaques spanning the adult lifespan, including exceptionally old individuals establishes a foundational framework for understanding the cellular and regulatory architecture of primate brain aging and its links to disease.
This review examines the experimental and computational foundations of scLR-seq, including platform selection, library design, cell barcode and unique molecular identifier recovery, transcript discovery, and isoform quantification, and summarize emerging insights into isoform usage, alternative splicing, transcription start and end site selection, allele-specific expression, fusion transcripts, transposable element-derived transcripts, and RNA modifications.
