Heterologous engineering of receptors using OrthoRep (HERO) for directed evolution of GPCRs in yeast
Engineering G protein-coupled receptors (GPCRs) for biosensing applications remains challenging due to structural and functional constraints when such proteins are expressed in a heterologous host. OrthoRep offers the continuous accumulation and selection of signal-enhancing mutations in yeast, yet it has not been appl...
Christien Dykstra, Björn D. M. Bean, Olivier Rousseau et al.
· bioRxiv (Cold Spring Harbor... · 0 citations