Cayden Williamson

Hydrogel compartmentalization enables flow cytometric selection of engineered yeast producing complex natural products.

Saccharomyces cerevisiae is a powerful chassis for biosynthesis of plant-derived natural products, but optimizing multi-enzyme pathways remains bottlenecked by the lack of scalable screens for small molecules with weak to no fluorescence. Here we present a colony-level, fluorescence-activated cell sorting (FACS) workfl...