Open access
Sep 2026
Development and evaluation of a two-tube multiplex real-time PCR assay for differential detection of Monkeypox virus clades Ia, Ib, IIa, and IIb
Monkeypox virus (MPXV) has caused two Public Health Emergencies of International Concern since 2022, with co-circulation of distinct clades (Ia, Ib, IIa, IIb) that differ in virulence and transmission patterns. Rapid discrimination of these clades is critical for outbreak control and clinical management. Here, we devel...
Xiang-Yu Zhu, Ning Shi, Yong Liu et al.
· Frontiers in Microbiology · 0 citations