Blagoy Spasov Blagoev

Chemogenetic ubiquitin E1 inhibitor TAK-243 CRISPR screen uncovers HAPSTR1/HUWE1 axis in chromatin proteostasis and cell cycle control

In this study, we sought to comprehensively map ubiquitin signaling networks at the crossroads of cellular proliferation and proteostasis, both crucial in cancer. Genome-wide CRISPR knockout screening in the presence of the ubiquitin E1 inhibitor TAK-243 identified 239 genes whose ablation sensitizes cells to ubiquitin...

Multi-organ proteomic atlas of obesity regression in male mice

UbiSite Approach: Global Site-Specific Profiling of Canonical and Non-Canonical Ubiquitination in Cells and Tissues.

The UbiSite approach, a refined strategy for site-specific mapping of global ubiquitination using mass spectrometry-based proteomics, offers a robust and versatile platform for comprehensive ubiquitinome profiling with enhanced specificity and depth, enabling new insights into the regulatory roles of ubiquitin across d...

SpY-C: Supervised Learning of Phosphopeptide Sequence Constraints Enables Global Prediction of SH2 Domain Binding

SpY-C is used as a classifier to annotate the human phosphoproteome and individual experiments, to explore the consequences of using super-SH2 domain reagents for pY enrichment, and to analyze the effects of mutations in altering pY site function.