Single-cell transcriptomics of cell-cell complexes offers key insights into in vivo immune interactions, yet distinguishing true physical contacts from artifacts remains challenging. Here, we integrated spectral imaging cell sorting with physical cell deconvolution to separate circulating T cell-monocyte doublets from...
Ning-Xin Kang, Cheryl Kim, Thomas J. Scriba et al.· bioRxiv· 0 citations
Circulating cell-cell complexes (doublets) provide critical insights into in vivo immune interactions. Here, we leverage high-dimensional imaging spectral cytometry to systematically map the landscape of immune doublets in human peripheral blood. By integrating morphometric imaging features with a 22-color spectral pan...
Cheryl Kim, Denise Hinz, Aaron Middlebrook et al.· bioRxiv· 0 citations
This study used single-cell RNA sequencing to provide a high-resolution characterization of CD4−CD8− γδ T cells in peripheral blood across healthy Mtb-(non-)sensitized and TB disease pre-/post-treatment cohorts and found an activated and cytotoxic gene signature in γδ T cells of TB disease compared with both healthy co...
Kendall Kearns, R. Tippalagama, Ashu Chawla et al.· iScience· 0 citations
A CDM for the Adaptive Immune Receptor Repertoire Knowledge Commons was designed based upon the Ontology for Biomedical Investigations, a community standard for scientific data integration, and the LinkML data modeling language was used for implementation.
Scott Christley, Felix Breden, Kevin A. Burns et al.· Journal of Immunology· 0 citations
Using the recently commercialized FACSDiscoverTM S8 spectral imaging cell sorter, image-based cell sorting provides unprecedented granularity in the study of immune doublets and enrichment for biological doublets over technical artifacts.
Ning-Xin Kang, Cheryl Kim, Thomas J. Scriba et al.· Journal of Immunology· 0 citations
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