Billy Wai-Lung Ng

Chemically Induced Proximity for Targeted Protein O- GlcNAcylation

O-GlcNAcylation is a ubiquitous post-translational modification across the mammalian proteome, yet determining how individual O-GlcNAcylation events control specific protein functions remains a central challenge. The O-GlcNAc cycle is governed by two enzymes: O-GlcNAc transferase (OGT), which installs O-GlcNAc, and O...

Targeted Control of Protein O-GlcNAcylation in Living Cells.

O-linked N-acetylglucosamine glycosylation (O-GlcNAcylation) regulates many intracellular proteins, but linking a cellular phenotype to O-GlcNAcylation of a specific substrate remains difficult. Global perturbation of O-GlcNAc transferase (OGT) or O-GlcNAcase (OGA) changes many proteins at once and therefore provides l...

Proximity-induced protein deglycosylation by endogenous O-GlcNAcase

Molecular Editing of Bilobalide: Expanding Chemical Space and Biological Opportunities

Complex natural products often show promising biological activity but suffer from liabilities, such as chemical or metabolic instability, that can limit their therapeutic evaluation. This perspective highlights bilobalide as a case study for how targeted molecular editing can transform a fragile, structurally dense n...

Targeted O-GlcNAcylation enables functional rewiring of c-Myc.

An O-GlcNAcylation-targeting Chimera (OGTAC) is developed that recruits O-GlcNAc transferase (OGT) to the oncogenic transcription factor c-Myc, enabling targeted O-GlcNAcylation in living cells without globally perturbing cellular O-GlcNAcylation.