Benjamin Vermeer

Nanophotonic DyeCycling delivers single-molecule FRET beyond photobleaching to identify heterogenous dynamics in DNA and protein systems

With its unique spatiotemporal resolution at the single-molecule level, Förster resonance energy transfer (FRET) is a powerful tool for investigating biomolecular conformational dynamics and function. However, early photobleaching remains a key limitation restricting the achievable observation time and therefore the in...