Tracking Surface Protein Assembly and Peptidoglycan Biosynthesis in Fusobacterium nucleatum.
A fluorescence-based labeling approach using fluorescent D-amino acids (FDAAs) to simultaneously track peptidoglycan biosynthesis and the localization of FadA in F. nucleatum is presented, providing a versatile platform for investigating the dynamic interplay between virulence factor localization and cell envelope remodeling.