Aidan E. Cowan

Replacing anaerobic fermentation with reverse β-oxidation enables growth-coupled evolution for enhanced fatty-alcohol production

Engineering robust fermentation hosts remains a barrier to scalable biomanufacturing. In this work we replace canonical mixed-acid fermentation in Escherichia coli with a redox‑balanced reverse β‑oxidation (rBOX) pathway that produces medium‑chain fatty alcohols and is bioenergetically equivalent to ethanol ferme...