Author

Ahmed Saleh

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Open access Aug 2026

Compact type II-C Cas9 nucleases with expanded PAM access and high fidelity for therapeutic genome editing

Three previously uncharacterized compact type II-C Cas9 orthologs are identified, and all show a strong deletion-biased repair signature and no detectable editing across 33 predicted off-target sites, expanding the CRISPR targeting space for AAV-deliverable therapeutic editing.

Qiaochu Wang, Sivakrishna Rao Gundra, Rashid Aman et al. · bioRxiv · 0 citations

Open access Aug 2026

Compact type II-D Cas9 nucleases for efficient and specific genome editing

Five previously uncharacterized MG102-like Cas9d orthologs are identified that share the hallmark genomic, sequence, and structural features of type II-D Cas9 and establish compact MG102-like Cas9d orthologs as robust and specific genome editors and provide promising, single-AAV– compatible scaffolds for in vivo therap...

Qiaochu Wang, Ahmed Saleh, G. S. Rao et al. · bioRxiv · 0 citations