A. Kaczmarczyk

A self-regulating dCas9 system enables robust, portable and multiplexed CRISPR interference

CRISPR interference (CRISPRi) enables programmable and reversible gene repression but often suffers from leakiness in the uninduced state, thereby confounding phenotypes of essential or dosage-sensitive genes. Here, we introduce a novel CRISPRi architecture, in which dCas9 restricts its own expression through a feedbac...